Detection of chromosomal aberrations in cytologic brush specimens from head and neck squamous cell carcinoma
✍ Scribed by Veltman, Joris A. ;Hopman, Anton H.�N. ;Bot, Fredrik J. ;Ramaekers, Frans C.�S. ;Manni, Johannes J.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 206 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
Background:
Detection of genetic changes in the mucosa of the upper aerodigestive tract may provide a target for the screening of cytologic specimens to identify premalignant transformation in this region. in this pilot study, the feasibility of the fluorescence in situ hybridization (fish) technique to detect genetically aberrant cells in brush specimens was evaluated.
Methods:
Brush specimens taken from the tumors of 20 patients with head and neck squamous cell carcinoma (hnscc) and from the normal mucosa of 8 control patients were analyzed by fish using dna probes for the chromosomes 1 and 7. the fish results were compared with dna flow cytometry and fish results of the solid tumor specimens.
Results:
The results of this study showed that 15 of the 20 tumor brush specimens contained numeric chromosomal aberrations in at least 5% of the cells collected. chromosomal aberrations were detected in all brush specimens taken from tumors that were dna aneuploid and showed aneusomy. the presence of these aberrations correlated well with the classification "suspicious for malignancy," which was based on papanicolaou stained slides of the same specimens. in the control group the percentage of chromosomally aberrant cells did not exceed 2%; in addition, no suspiciously malignant cells were observed in this group.
Conclusions:
This study reveals that the fish technique can be applied diagnostically to brush specimens of hnscc. the presence of chromosomal aberrations in > 5% of the cells in these specimens can be considered as a marker for malignancy.
📜 SIMILAR VOLUMES
The MAGE-4 gene, a member of the MAGE gene family, is expressed in various cancers, including head-and-neck squamous-cell carcinomas (HN-SCC), but is not expressed in any normal tissues except for the testis and placenta. The aim of this study was to determine whether serum MAGE-4 protein is a usefu
Several lines of evidence suggest that the progression of head-and-neck squamous-cell carcinoma (HNSCC) involves inactivation of at least one and possibly several tumorsuppressor genes on the long arm of chromosome 13. The fact that neither Rb1 nor BRCA2 appears to be inactivated in the majority of
Loss of wild-type p53, either through deletion or mutation, has been demonstrated in most squamous cell carcinomas of the head and neck (HNSCC). Whether these mutant molecules contribute to tumor progression purely through loss of wild-type functions or by growth-promoting mechanisms, however, remai
## Background: Abnormalities of chromosome band 11q13 are frequent in squamous cell carcinoma of the head and neck (scchn). the oncogene ccnd1 is located at 11q13 and encodes cyclin d1, a cell cycle-regulating protein. the authors investigated the clinical relevance and associations between amplifi
## BACKGROUND. The authors previously have found that in patients with locally advanced squamous cell carcinoma of the head and neck (SCC-HN), alternating chemoradiotherapy (ALT) was superior to low-total-dose conventional radiotherapy alone. The purpose of this randomized trial was to compare the