Highly purified myelin from rat brain stem has been shown to contain phosphatidate phosphohydrolase, an enzyme which converts phosphatidate to diacylglycerol. The high levels relative to cytosol and microsomes (17% and 22%, respectively) tended to preclude contamination by these fractions as the sou
β¦ LIBER β¦
Detection of choline kinase in purified rat brain myelin
β Scribed by Tatsuhide Kunishita; Kuldeep K. Vaswani; Charles R. Morrow; Robert W. Ledeen
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 511 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0364-3190
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Using a monoclonal antibody (P6C3) derived from mice immunized with partially delipidated rat myelin, we detected a single 57.5 kDa protein species (BT57.5) positioned between a-and fi-tubulin on nitrocellulose containing electrophoretically separated rat brain myelin proteins. The kinetics of incor