𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of cellular immunity to tumor-associated antigen(s) in mice by the macrophage spreading inhibition test

✍ Scribed by Ivna Svoboda; Renata Mažuran; Sabina Rabatić; V. Silobrčić

Publisher
John Wiley and Sons
Year
1977
Tongue
French
Weight
699 KB
Volume
19
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Soluble preparations of antigens from a methyl‐cholanthrene‐induced fibrosarcoma of C57BI mice were prepared by homogenization of tumor tissue and high‐speed centrifugation of the homogenate. These preparations were able to sensitize syngeneic mice to tumor‐associated antigens (TAA) of the fibrosarcoma, and to provoke a delayed hypersensitivity reaction when injected into the footpad of sensitized mice. Furthermore, the same soluble preparations could inhibit in vitro the spreading of peritoneal macrophages from mice sensitized to TAA. A similar inhibition of macrophage spreading was obtained when peritoneal cells from C57BI mice, bearing transplants of the fibrosarcoma, were incubated with the preparations. We conclude that the macrophage spreading inhibition test, like other in vitro assays, can detect cell‐mediated immunity to tumor‐associated antigens.

📜 SIMILAR VOLUMES

In vitro detection of cellular immunity
In vitro detection of cellular immunity to melanoma antigens in man by the monocyte spreading inhibition test
✍ Renata Mažuran; H. Mujagic; B. Malenica; V. Silobrčic 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 French ⚖ 581 KB

## Abstract In vitro inhibition of monocyte spreading (a correlate of cellular immunity) was used to detect cell‐mediated immune reactions of melanoma patients to specific melanoma antigens. Two soluble preparations of human melanoma antigens (MA‐1 and MA‐2) and one of a breast carcinoma (BCA) were