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Detection of carbonic anhydrase 9–expressing tumor cells in the lymph nodes of vulvar carcinoma patients by RT-PCR

✍ Scribed by Magdalena Kowalewska; Jakub Radziszewski; Jadwiga Kulik; Monika Barathova; Anna Nasierowska-Guttmajer; Mariusz Bidziński; Jaromir Pastorek; Silvia Pastorekova; Janusz A. Siedlecki

Publisher
John Wiley and Sons
Year
2005
Tongue
French
Weight
145 KB
Volume
116
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Regional lymph node status is an important prognostic factor for vulvar cancer. The goal of our study was to elaborate a reliable test for detecting micrometastases, undetectable by traditional methods, in the lymph nodes of vulvar squamous carcinoma patients. For this purpose, carbonic anhydrase‐9 (CA9) was investigated as a cancer‐related marker by RT‐PCR. Firstly, primary carcinoma specimens were examined for CA9 expression by immunohistochemistry with M75 monoclonal antibody. All 19 tissues exhibited a variable degree of staining, which was mostly confined to the plasma membranes of tumor cells. Correspondingly, all primary tumor specimens and the control A‐431 vulvar cancer cell line gave a positive signal in the nested RT‐PCR assay designed to detect CA9‐expressing cells with a high sensitivity. Analysis of 77 lymph node specimens from 20 patients revealed a full correlation between RT‐PCR results and standard hematoxylin–eosin staining in 75% of samples, whereas 25% of specimens were negative by the standard method and positive for CA9 mRNA, accounting for 28% of all histologically negative lymph nodes. There were no false‐negatives with RT‐PCR. A positive inguinal lymph node with a negative sentinel node was observed in the same groin only once in 38 specimens. Our findings clearly indicate potential value of CA9 as a molecular marker for the assessment of regional lymph node status in vulvar cancer patients and support a possible utility of our RT‐PCR assay in the detection of micrometastases. © 2005 Wiley‐Liss, Inc.

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