Detection of Candida albicans mannan by immunodiffusion, counterimmunoelectrophoresis, and enzyme-linked immunoassay

✍ Scribed by Paul F. Lehmann; Errol Reiss

• Publisher: Springer Netherlands
• Year: 1980
• Tongue: English
• Weight: 631 KB
• Volume: 70
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00443072

✦ Synopsis

Anti-mannan was produced in rabbits after peptidoglucomannan in adjuvant was injected. The antiserum was used to detect mannan by immunodiffusion and counterimmunoelectrophoresis (CIE) in gel and by sandwich enzyme-linked immunosorbent assay (ELISA). The antiserum detected lower concentrations of mannan of serotype A than of serotype B. Except in CIE, the reactions were more pronounced at 4 ~ than at higher temperatures. CIE detected 0.8 #g/ml mannan A or 12.5/~g/ml mannan B. Sandwich ELISA detected 3 ng/ml mannan A or l0 s ng/ml mannan B. Mannan was not detected in the serum of patients or rabbits with candidiasis.