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Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with lyme arthritis

✍ Scribed by Benoǐt Jaulhac; Isabelle Chary-Valckenaere; Jean Sibilia; Rose-Marie Javier; Yves Piémont; Jean-Louis Kuntz; Henri Monteil; Jacques Pourel

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
1002 KB
Volume
39
Category
Article
ISSN
0004-3591

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✦ Synopsis

Objective. To compare the detection rates of chromosomal flagellin gene from Borrelia burgdotfen' in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques.

Methods. B burghgen' DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls).

Results. No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdotfen' DNA was detected in all ST andlor SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the ll remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05).

Conclusion. These data suggest that detection of chromosomal B burgdorfen' DNA may be more efficient in ST than SF.

The diagnosis of Lyme arthritis, a frequent manifestation of Lyme disease, may be difficult when typical features, such as tick bite or erythema chronicum migrans (ECM), are absent or when the symptoms mimic those of another rheumatic disease. The causative agent of Lyme arthritis, Borrelia burgdorferi (B burgdorferi), has proven to be difficult to culture from synovial samples. Indeed, culture is rarely successful; only 2 strains have thus far been isolated from

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