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Detection of biotinylated nucleic acid hybrids by antibody-coated gold colloid

✍ Scribed by Stephen Tomlinson; Andrew Lyga; Edward Huguenel; Nanibhushan Dattagupta

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
650 KB
Volume
171
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and sensitive nonradioactive method for detecting biotinylated nucleic acid hybrids on nitrocellulose paper is described. The method employs anti-biotin antibody-coated gold colloid and silver enhancement. Comparative results indicate sensitivity similar to that of a commonly used enzymatic colorimetric detection method.

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