For diagnosis of HIV-1 infection, attempts were made to detect anti-HIV-1 IgG in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) US- ing recombinant reverse transcriptase (RT) and p17 as antigens. Anti-HIV-1 IgG in urine was reacted simultaneously with 2,4-dinitrop
Detection Of Autoantibodies To Recombinant Human Thyroid Peroxidase By Sensitive Enzyme Immunoassay
โ Scribed by D. L. Kendler; A. Martin; R. P. Magnusson; T. F. Davies
- Book ID
- 114719219
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 576 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0300-0664
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Recombinant HIV-1 p17 antigen (rp17) and maltose binding protein-rp17 fusion protein (MBP-rp17) were immobilized in different ways: rp17 and MBP-rp17 were immobilized directly onto polystyrene beads by physical adsorption, and biotinyl-rp17, biotinyl-MBP-rp17, and 2,4-dinitrophenyl (DNP)-MBP-rp17 we
Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT), p i 7 and p24 as antigens, and p-o-galactosidase from Escherichia coli as label. Anti-HIV-I IgG in urine was reacted simultaneously