Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe
✍ Scribed by Udo Trautmann; Gabriele Leuteritz; Gabriele Senger; Uwe Claussen; Wolfgang G. Ballhausen
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 363 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
The chromosome region 5q22 harbouring the putative gene associated with adenomatous polyposis coli (APC) was microdissected and microcloned from GTG-banded human metaphase chromosomes. In order to determine the precise regional localization of the microdissected material, we used polymerase chain reaction amplified microclones as a bulk-probe in nonradioactive chromosomal in situ suppression hybridization of human metaphase spreads. Specific in situ hybridization signals were obtained on the long arm of chromosome 5 in accordance with the chromosomal region excised for the cloning procedure. The application of this detection system should provide a rapid and powerful tool for analyzing patients with translocations or microdeletions of a given chromosome region.