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Detection of APC mutations in stool DNA of patients with colorectal cancer by HD-PCR

✍ Scribed by Rainer Deuter; Oliver Müller

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
204 KB
Volume
11
Category
Article
ISSN
1059-7794

No coin nor oath required. For personal study only.

✦ Synopsis

In most cases the analysis of DNA mutations in presence of a high excess of wild type DNA fail because of the low sensitivity of the performed method for mutation detection. Here we describe the new high-sensitive and non-radioactive HD-PCR method (for HeteroDuplex-PCR). In opposite to the conventional analytical application the heteroduplex technique is performed to preparatively separate mutated from non-mutated PCR amplified DNA fragments. We used the new method to detect mutations in the tumor suppressor gene APC in stool DNA from patients with sporadic colorectal carcinomas. Since the alteration of the APC gene occurs early in most colorectal tumors, the detection of APC mutations in fecal tumor DNA by HD-PCR may be a powerful tool in non-invasive cancer diagnostics.

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