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Detection of anticentromere antibodies using cloned autoantigen cenp-Bl

โœ Scribed by N. Rothfield; D. Whitaker; B. Bordwell; E. Weiner; J.-L. Senecal; W. Earnshaw

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
401 KB
Volume
30
Category
Article
ISSN
0004-3591

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โœ Dongxu Sun; Antigona Martinez; Kevin F. Sullivan; Gordon C. Sharp; Sallie O. Hoc ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 503 KB

## To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by e