Detection of antibodies to single-stranded dna in naturally acquired and experimentally induced viral hepatitis

✍ Scribed by Ian D. Gust; Stephen M. Feinstone; Robert H. Purcell; Harvey J. Alter

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 601 KB
• Volume: 6
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890060102

✦ Synopsis

Abstract

A sensitive “Farr” assay, utilizing ^125^ I‐labelled DNA was developed for detecting antibody to single‐stranded DNA (anti‐ssDNA). The test was shown to be specific and as sensitive as assays using ^14^C‐labelled DNA, for the detection of antibody in patients with connective tissue diseases.

Groups of sera from patients with naturally acquired viral hepatitis and experimentally infected chimpanzees were tested for anti‐ssDNA by the ^125^I assay and by counterimmunoelectrophoresis (CIEP). No consistent pattern was observed with either technique, indicating that elevated levels of this antibody are not as reliable markers of parenchymal liver damage as had been previously suggested.