Detection of an insensitive ornithine-carbamoyltransferase in strains of Pseudomonas syringae pv. phaseolicola with different phytotoxin-generating capacities

✍ Scribed by O. Jahn; J. Sauerstein; F. Laplace; Prof. Dr. G. Reuter

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 301 KB
• Volume: 29
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620290517

✦ Synopsis

Independently of their capacity to produce phytotoxins. strains of Pseudornonas syringae pv. phaseolicola contain two ornithine carbamoyltransferases (OCT, EC 2.1.3.3.) which differ in resistance to phaseolotoxin and Orn-P(0) (NH&NH-SO, H (PNSOrn). At 18 "C, the optimal temperature for product formation, the balance of the two types of OCT was shifted in favour of the insensitive type in phaseolotoxin producing strains, and in favour of the sensitive one in strains with little or no toxin production. The results suggest a causal relationship between the existence of an insensitive enzyme and the synthesis of toxins.