✦ LIBER ✦

Detection of aberrations in androgen receptor gene by analysis of single-stranded conformation polymorphisms in polymerase chain reaction products

✍ Scribed by Kondoh, N. ;Namiki, M. ;Takahara, S. ;Takada, S. ;Kitamura, M. ;Koh, E. ;Matsumiya, K. ;Kiyohara, H. ;Okuyama, A.

Publisher: Springer
Year: 1995
Tongue: English
Weight: 621 KB
Volume: 23
Category: Article
ISSN: 0300-5623
DOI: 10.1007/bf00393303

No coin nor oath required. For personal study only.

✦ Synopsis

Analysis of single-stranded conformation polymorphisms in polymerase chain reaction (PCR) products (PCR-SSCP) is a sensitive method for detecting point mutations in genomic DNA. To investigate its utility in examining the androgen receptor gene, we analyzed data on a patient with the testicular feminization syndrome (TFS) with a known point mutation in exon C. We detected mobility shifts of fragments of the corresponding region. Since examination of the subject's brother (legally sister), who also has TFS, revealed an identical shift pattern, we sequenced the exon C of the sibling and detected a mutation identical to that in the former. We conclude that PCR-SSCP is available for screening mutations of the androgen receptor gene.

📜 SIMILAR VOLUMES

Polymerase chain reaction detection of a

Polymerase chain reaction detection of a novel human KIT (mast/stem cell growth factor receptor) gene polymorphism by single-strand conformation polymorphism analysis or bySmaI orBstNI cleavage

✍ Richard A. Spritz; Stuart A. Holmes 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 289 KB

Low incidence of androgen receptor gene

Low incidence of androgen receptor gene mutations in human prostatic tumors using single strand conformation polymorphism analysis

✍ Evans, B.A.J.; Harper, M.E.; Daniells, C.E.; Watts, C.E.; Matenhelia, S.; Green, 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 882 KB

It is possible that structural changes of the androgen receptor (AR) contribute to the insensitivity of prostatic carcinomas to endocrine therapy. We have isolated DNA from 58 human prostate tumor specimens (31 carcinomas pretreatment, 13 carcinomas after relapse to hormonal therapy, and 14 benign p

Polymerase chain reaction detection of t

Polymerase chain reaction detection of two novel human N-acetylgalactosamine-6-sulfate sulfatase gene polymorphisms by single-strand conformation polymorphism analysis or byStyI andStuI cleavages

✍ Shunji Tomatsu; Seiji Fukuda; Atsushi Uchiyama; Toshinori Hori; Yoshinori Nakash 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 403 KB

Mobility of Digoxigenin-dUTP Polymerase

Mobility of Digoxigenin-dUTP Polymerase Chain Reaction Products and Detection of Single-Stranded Products in Agarose Electrophoresis

✍ M. Nagai; T. Nemoto; M. Ota 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 353 KB

Rapid detection of sequence polymorphism

Rapid detection of sequence polymorphisms in the human mitochondrial DNA control region by polymerase chain reaction and single-strand conformation analysis in mutation detection enhancement gels

✍ Dr. Antonio Alonso; Pablo Martin; Cristina Albarran; Oscar Garcia; Manuel Sancho 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB 👁 1 views

Detection of mutations and polymorphisms

Detection of mutations and polymorphisms in the p53 tumor suppressor gene by single-strand conformation polymorphism analysis

✍ Laura S. Kutach; Svetlana Bolshakov; Honnavara N. Ananthaswamy 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 124 KB 👁 2 views

## Detection of mutations and polymorphisms in the p53 tumor suppressor gene by single-strand conformation polymorphism analysis Deciphering the genetic mechanisms in cancer development requires analysis of a large number of tumors for consistent genetic alterations. Single-strand conformational p