Detection of a unique γ-glutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: A new candidate for early diagnosis of hepatocellular carcinoma

✍ Scribed by M Tsutsumi; D Sakamuro; A Takada; S Zang; T Furukawa; N Taniguchi

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 158 KB
• Volume: 23
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.510230524

✦ Synopsis

sues with HCC. In noncancerous tissues of livers with Many studies concerning g-glutamyl transpeptidase HCC, the main types were types A and B. The prevalence (GGTP) in hepatocellular carcinoma (HCC) have sugof type B was significantly higher in both cancerous and gested that changes in hepatic GGTP expression may be noncancerous tissues of livers with HCC than in livers closely related to the development of HCC. However, its without HCC. The prevalence of type A in cancerous mechanisms are not well known, and genomic analysis tissue, but not in noncancerous tissue, was significantly of the specific GGTP to HCC is also lacking. Recently, lower than in livers without HCC. These results strongly the human GGTP complementary DNA (cDNA) sesuggested that the GGTP mRNA expression in human quences from fetal liver, placenta, and HepG2 cells have liver may shift from type A to type B during the developbeen published. In the present study, we sought to clarment of HCC. The high prevalence of type B in noncanify the distribution of the GGTP mesenger RNA (mRNA) cerous tissues suggested that the shift of the GGTP molecular species in human liver and determine mRNA may occur from the preneoplastic stage of hepawhether alterations in GGTP mRNA expression occur tocytes. (HEPATOLOGY 1996;23:1093-1097.) upon the development of HCC. The specific primer sets for reverse-transcription polymerase chain reaction (PCR) corresponding to the 5-noncoding human GGTP g-Glutamyl transpeptidase (GGTP) is a plasma mRNA of fetal liver (type A), HepG2 cells (type B), and membrane-bound enzyme of major importance in the placenta (type C) were prepared. Oligonucleotide probes metabolism of glutathione. It has been reported that specific for each type of mRNA were also synthesized. hepatocellular carcinoma (HCC) in both rats and hu-Liver tissues were obtained from patients with or withmans expresses GGTP enzymes with unique carbohyout HCC, and total RNA was extracted. Total RNA was drate moieties compared with normal liver enzymes.

also extracted from various organs obtained from one

The presence of the unique GGTP isoform for HCC in male patient upon autopsy. Types of GGTP mRNAs were analyzed using type-specific primer sets and oligonucle-patient sera was used as a marker for the diagnosis of otide probes. The types of GGTP mRNA varied in differ-HCC. 4,5 GGTP was used as an important marker enent organs. In normal liver and diseased liver without zyme for chemically induced HCC, because it is induced HCC, the main type of GGTP mRNA was type A. The in primary HCC and preneoplastic lesions of the liver. 6 expression was monogenic in most cases but was poly-Mallory bodies, one of the hallmarks of alcoholic liver genic in some cases. In the polygenic cases, type C was injury, are also found in the liver with HCC. Expericommon, but type B was found occasionally. On the mentally, Mallory bodies develop in mice treated chronother hand, type B was predominant in cancerous tisically with gliseofulvin, and HCC is also found in these animals. In hepatocytes developing Mallory bodies, histologically detectable GGT activity was observed from Abbreviations: GGTP, g-glutamyl transpeptidase; HCC, hepatocellular carthe early stage of development. These results cinoma; cDNA, complementary DNA; mRNA, messenger RNA; PCR, polymerstrongly suggested that changes in GGTP in livers may ase chain reaction.