Detection of a nuclear antigen 2 (EBNA2)-variant Epstein-Barr virus strain in two siblings with fatal lymphoproliferative disease
✍ Scribed by Schuster, Volker; Seidenspinner, Silvia; Kreth, Hans Wolfgang
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 829 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
An EBV type 1 variant strain was detected in two Turkish siblings (boy and girl), who both suffered and died from similar progressive Epstein-Barr virus (EBV)-associated lymphoproliferative disease. Molecular characterisation of this EBV isolate revealed a 51 bp-deletion and six nucleotide changes within the Epstein-Barr nuclear antigen 2 (EBNA2). Both isolates contained EBV type 2 sequences in the Epstein-Barr virus-encoded small RNAs (EBER), which are 40 kb proximal to EBNA2. Sequencing of the EBV isolates in a region of Epstein-Barr nuclear antigen 3 (EBNA3a1, which is 40 kb distal to EBNA2, revealed the normal EBV type 1 sequence of laboratory strain 695-8. This EBV isolate may represent a distinct wild type EBV strain with altered biological properties. It is suggested that this EBNA2-variant strain may be responsible at least in part for the severe clinical course in both affected children.