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Detection of a novel DNA virus (TTV) sequence in peripheral blood mononuclear cells

✍ Scribed by Okamura, Akiko; Yoshioka, Mikio; Kubota, Mitsuru; Kikuta, Hideaki; Ishiko, Hiroaki; Kobayashi, Kunihiko

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 153 KB
Volume: 58
Category: Article
ISSN: 0146-6615
DOI: 10.1002/(sici)1096-9071(199906)58:2<174::aid-jmv12>3.0.co;2-x

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✦ Synopsis

DNA sequences of a novel DNA virus (TTV) were examined in 81 peripheral blood mononuclear cell (PBMC) DNA samples from 48 children and 33 adults, 22 cord blood mononuclear cells (CBMC) DNA samples, and 7 autopsy liver tissue DNA samples by a hemi-nested polymerase chain reaction (PCR). The PCR was carried out using the published primers (NG059, NG061, NG063) to amplify TTV DNA sequences. The sequences were detected in 4 of 81 (5%) PBMC DNA samples, in none of 22 (0%) CBMC DNA samples, and in 2 of 7 (29%) liver tissue DNA samples by direct gel analysis. The PCRamplified products were confirmed by direct sequencing. The sequencing showed considerable diversities, with differences of 0-55% in 6 TTV isolates, compared with the prototype sequence of TTV. These results suggest that TTV is a ubiquitous virus that produces asymptomatic infection in a large proportion of the general population without transfusion of blood-derived products. To our knowledge, this is the first report describing the detection of TTV DNA sequences in PBMCs.

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