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Detection of a new polymorphism of the human prothrombin (F2) gene by combination of PASA and mutated primer-mediated PCR-RFLP

✍ Scribed by Hiroyuki Iwahana; Noriko Mizusawa; Katsuhiko Yoshimoto; Mitsuo Itakura

Publisher: Springer
Year: 1992
Tongue: English
Weight: 244 KB
Volume: 90
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00220094

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✦ Synopsis

A new polymorphism of the human prothrombin (F2) gene was detected by a combination of polymerase chain reaction (PCR) amplification of specific alleles (PASA) and mutated primer-mediated PCR restriction fragment length polymorphism (PCR-RFLP). The method is simple and useful for detecting polymorphisms and mutations. The new polymorphism of C1 and C2 examined by this method is highly heterozygous and serves as a good human DNA marker.