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Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time PCR without enrichment

โœ Scribed by Jung-Lim Lee; Robert E. Levin


Book ID
113625710
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
283 KB
Volume
28
Category
Article
ISSN
0740-0020

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t