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Detection of 15N-labeled ammonium. In Vivo, 15N NMR versus mass spectrometry

✍ Scribed by G.G Fox; R.G Ratcliffe; S.A Robinson; A.P Slade; G.R Stewart

Book ID
107798629
Publisher
Elsevier Science
Year
1992
Weight
484 KB
Volume
96
Category
Article
ISSN
0022-2364

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For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryp