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Detection of (1–3)-β-D-glucan in a rat model of aspergillosis

✍ Scribed by Kotaro Mitsutake; Shigeru Kohno; Takashige Miyazaki; Yoshihiro Yamamoto; Katsunori Yanagihara; Hiroshi Kakeya; Atsuro Hashimoto; Hironobu Koga; Kohei Hara

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
372 KB
Volume
9
Category
Article
ISSN
0887-8013

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✦ Synopsis

The G test containing factor G, fractioned from the Lirnulus lysate, was used to detect (1-3)-b-o-glucan in a rat model of aspergillosis. Aspergillus furnigatus strain MF-13, l x 1 0 4 conidia, were inoculated transtracheally into rats treated with cortisone acetate (100 mg/kg) and fed a low-protein (8%) diet. Increased serum (1-3)-p-oglucan was found on the sixth day after inoculation in concentrations of 370 2 178 pg/ ml (mean &D) in untreated controls, and 154 2 43 pg/ml in rats treated with 0.5 mg/ kg of amphotericin B. On day 11 (1-3)-P-oglucan concentrations were 2,590 2 2,940 pg/ml and 448 -+ 442 pg/ml, respectively. The elevation in levels of (1-3)-p-o-glucan increased in correlation with the elevation of galactomannan antigen titers; (1-3)-p-oglucan is thus measurable during experimental aspergillosis in rats. 01995 ~i ~e y -~i s s , Inc.

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