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Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus

✍ Scribed by Tarek Zmantar; Kamel Chaieb; Héla Makni; Hanene Miladi; Fethi Ben Abdallah; Kacem Mahdouani; Amina Bakhrouf

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
263 KB
Volume
48
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to detect the adhesins genes (ica A, ica D, fnb A, cna, Clf A) Polymerase Chain Reaction was used. Qualitative biofilm production of S. aureus using CRA plates revealed that 56.5% of strains were slime producers. In addition 78.26% of strains were ica A and ica D positive. While the fnbA gene was present in 76.1% of isolated strains. Furthermore, 56.5% of strains have the cna gene and 30.4% were clfA positives. Overall this study confirms the presence of fnb A and ica A/ica D genes in the majority of studies S. aureus strains isolated from Staphylococcal sepsis. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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