Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims

✍ Scribed by Hendrix, M. G. Ron; Beuken, Eric; Slobbe, Rob L.; Bruggeman, Cathrien A.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 493 KB
• Volume: 50
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/(sici)1096-9071(199610)50:2<193::aid-jmv14>3.0.co;2-n

✦ Synopsis

The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV-seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. In one subject two types of the MIE-4 gene were detected, and in another subject two types of the pp150 gene were found, suggesting that different strains of HCMV can be found i n organs of the same patient during latency.