A cytometric method for detecting and quantitating hybrid cells that resulted from cell-cell electrofusion was developed. Cells from two different lines and two vital fluorescent dyes were used in conjunction with a flow cytometer to demonstrate the method. Each dye was used to stain one cell type p
Detection and quantitation of Bacteroides gingivalis in bacterial mixtures by means of flow cytometry
β Scribed by K. S. Kornman; M. Patters; R. Kiel; and P. Marucha
- Book ID
- 110107390
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 194 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0022-3484
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Prior to flow cytometric analysis, the cells were fixed in 70% ethanol and stained with a DNAspecific dye. After 1 h of ampicillin incubation, the light scattering and fluorescence intensities of the susceptible cells increased 4.3-fold and 5-fold, respectively, but remained about constant for the r
Clinical investigations utilizing murine monoclonal antibodies require techniques for the detection of the human anti-mouse antibody (HAMA) response in patient serum. We report here a flow cytometric assay for the quantitation of HAMA. Commercially available beads conjugated with goat anti-mouse ant