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Detection and quantification of Streptomyces violaceolatus plasmid DNA in soil

✍ Scribed by N. Cresswell; V.A. Saunders; E.M.H. Wellington

Book ID
115271789
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
318 KB
Volume
13
Category
Article
ISSN
0266-8254

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Two cryptic plasmids pMG110 (10.5 kb) and pMG120 (14.5 kb) isolated from Streptomyces luteolutescens were cleaved by restriction endonucleases Bg/II, KpnI, and SalGI. A physical map was constructed for pMG110. After denaturation and intrastrand reannealing, two types of snap-back structures were ide