Detection and quantification of goat's cheese in ewe's cheese using a monoclonal antibody and two ELISA formats
✍ Scribed by Haza, Ana I; Morales, Paloma; Mart�n, Rosario; Garc�a, Teresa; Anguita, Gonzalo; Sanz, Bernab�; Hern�ndez, Pablo E
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 79 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
A stable hybridoma cell line (B2B) has been produced secreting a monoclonal antibody (MAb) speci®c for the s a s2 -casein of goats. The MAb B2B was used in two enzyme-linked immunosorbent assays (ELISA) formats for the detection and quanti®cation of the presence of goat's cheese in ewe's cheese samples. In the indirect ELISA format the limit of detection was 1±25% (w/w) substitution of ewe's cheese samples by goat's cheese. Afterwards, a competitive indirect ELISA was successfully developed for the detection of 0.5 to 25% (w/w) of goat's cheese in ewe's cheese samples. This competitive indirect ELISA is a very sensitive assay, can be performed in less than 5 h and is not in¯uenced by the ripening process in cheese.