Detection and quantification of 5 enolpyruvylshikimate-3-phosphate synthase (cp4epsps) uponBrassica napus
โ Scribed by Xiaoling Song; Krysty Munns; Sheng Qiang; Robert Blackshaw; Ranjana Sharma
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 308 KB
- Volume
- 228
- Category
- Article
- ISSN
- 0044-3026
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๐ SIMILAR VOLUMES
5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.9) from the glyphosate-tolerant cyanobacterium Anabaena variabilis (ATCC 29413) was purified to homogeneity. The enzyme had a similar relative molecular mass to other EPSP synthases and showed
5-enolpyruvylshikimate-3-phosphate synthase (EPSPs), the target of the herbicide glyphosate, catalyzes an essential step in the shikimate pathway common to aromatic amino acid biosynthesis. We have cloned an EPSP synthase gene from Arabidopsis thaliana by hybridization with a petunia cDNA probe. The