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Detection and Purification of Tyrosine-sulfated Proteins Using a Novel Anti-sulfotyrosine Monoclonal Antibody

✍ Scribed by Hoffhines, A. J.; Damoc, E.; Bridges, K. G.; Leary, J. A.; Moore, K. L.


Book ID
127334912
Publisher
The American Society for Biochemistry and Molecular Biology
Year
2006
Tongue
English
Weight
942 KB
Volume
281
Category
Article
ISSN
0021-9258

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## Abstract We evaluated the potential for polyelectrolyte induced precipitation of antibodies to replace traditional chromatography purification. We investigated the impact of solution pH, solution ionic strength and polyelectrolyte molecular weight on the degree of precipitation using the anionic