Detection and morphologic evaluation of allotransplanted rat pancreatic islet cells
✍ Scribed by P. Fiedor; K. Szymanska; A. Tomaszewska; K. Socha; S.F. Oluwole; I. Licińska; A.P. Mazurek; W. Rowinski; M.A. Hardy
- Book ID
- 117234843
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 111 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0041-1345
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Islet cell lines were produced by retroviral transduction of SV-40 T antigen to monolayer cultures of neonatal rat islets. One line, RN0-11, showed evolution of phenotypes in vitro. It evolved from a non-hormone secreting cell line to an insulin secreting line. It further developed glucagon producin
Morphologically different pancreatic islet cells of the rat reveal immunoreactivity under incubation with anti-ACTH 1-39. In normal rats, anti-ACTH reactivity is detectable predominantly in peripheral, polygonal cells. Paraffin serial section treated alternately with anti-ACTH and anti-glucagon show