Detection of human orthopoxvirus infecti
Detection of human orthopoxvirus infections and differentiation of smallpox virus with real-time PCR
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Niina Putkuri; Heli Piiparinen; Antti Vaheri; Olli Vapalahti
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Article
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2008
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John Wiley and Sons
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English
β 231 KB
## Abstract We developed a realβtime PCR protocol to detect orthopoxviruses (OPVs) from different clinical specimens and to separate variola virus from other OPVs. In our protocol, we used automated nucleic acid extraction system together with realβtime PCR to create a simple, safe and fast procedu