✦ LIBER ✦

Detection and Identification of Ki-ras Exon 1 Mutations by Minigel Single-Strand Conformation Polymorphism

✍ Scribed by Arve Ulvik; Nina Karin Aarskog; Dagfinn Ogreid

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 51 KB
Volume: 232
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1995.0021

No coin nor oath required. For personal study only.

✦ Synopsis

Detection and Identification of Ki-ras Exon 1

The PCR mix consisted of 10 mM Tris -HCl, pH 9.0, Mutations by Minigel Single-Strand 50 mM KCl, 1.5 mM MgCl 2 , 0.01% (w/v) gelatin, 0.1%

Conformation Polymorphism

Triton, 125 mM of each dNTP, 0.2 mM of each primer and 0.2 U of Taq polymerase (Supertaq, HT Biotechnology Ltd., UK) in a total volume of 100 ml. The Arve Ulvik, Nina Karin Aarskog, and Dagfinn Ogreid product size was 221 bp.

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