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Detection and genotyping of bovine diarrhea virus by reverse transcription-polymerase chain amplification of the 5′ untranslated region

✍ Scribed by C Letellier; P Kerkhofs; G Wellemans; E Vanopdenbosch

Book ID
114246457
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
261 KB
Volume
64
Category
Article
ISSN
0378-1135

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## Abstract A reverse transcription‐polymerase chain reaction (RT‐PCR) was developed for the detection of __Chikungunya__ virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of __Chikungunya__, two primer sets were designed. Total RNA were extracted from the