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Detection and differentiation of Dengue infections with highly sensitive real-time PCRs

✍ Scribed by M. Kaiser; D. Bray; K. Benlhassan-Chahour; F. Bissuel; P. Huc Anais; W. Rozenbaum; H. Ellerbrok

Book ID
118451720
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
68 KB
Volume
14
Category
Article
ISSN
1201-9712

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## Abstract We developed a real‐time PCR protocol to detect orthopoxviruses (OPVs) from different clinical specimens and to separate variola virus from other OPVs. In our protocol, we used automated nucleic acid extraction system together with real‐time PCR to create a simple, safe and fast procedu