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Detection and characterization of endothelin in transformed human osteoblast cell culture medium

✍ Scribed by Hing-Chung Lam; Jenn-Kuen Lee; Kwok-Hung Lai


Book ID
111637520
Publisher
Springer
Year
2000
Tongue
English
Weight
86 KB
Volume
12
Category
Article
ISSN
0969-711X

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We characterized the effects of acetylcholine and endothelin on cultured human ciliary muscle cells, using the calcium-sensitive dye fura-2 to measure intracellular calcium and intracellular microelectrodes to measure the membrane potential. Both agonists, endothelin and acetylcholine, had a typical