Detection and analysis of urinary peptides by on-line liquid chromatography and mass spectrometry: application to patients with renal Fanconi syndrome
✍ Scribed by CUTILLAS, Pedro R.; NORDEN, Anthony G. W.; CRAMER, Rainer; BURLINGAME, Alma L.; UNWIN, Robert J.
- Book ID
- 120160011
- Publisher
- Portland Press
- Year
- 2003
- Tongue
- English
- Weight
- 163 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0143-5221
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An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a
## Abstract Advanced glycation end‐product (AGE)/peptides, arising from __in vivo__ digestion of glycated proteins, are biologically important compounds, due to their reactivity against circulating and tissue proteins. For information on their possible structure, __in vitro__ glycation of HSA and i