Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy
✍ Scribed by H. Bai; P. Chen; H. Fang; L. Lin; G.Q. Tang; G.G. Mu; W. Gong; Z.P. Liu; H. Wu; H. Zhao; Z.C. Han
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 248 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1612-2011
No coin nor oath required. For personal study only.
✦ Synopsis
Recent research suggests that human umbilical cord derived mesenchymal stem cells (hUC-MSCs) can be promising candidates for cell-based therapy. Since large population and high viability are generally required, detecting viability transitions of these cells is crucial for their population expansion and quality control. Here, as a non-invasive method, Raman micro-spectroscopy is applied to examine hUC-MSCs with different viability. Using peak fitting and statistic t-test, the Raman peaks with obvious differences between the cells with high viability (> 90%) and low viability (< 20%) are extracted. It is found that the C=O out of plane bending in thymine at 744 cm -1 , symmetric stretching of C-C in lipids at 877 cm -1 and CH deformation in proteins at 1342 cm -1 show the most significant changes (p < 0.001). When the cell viability decreases, the intensities of the former two peaks are both about doubled while that of the latter peak reduces by about 30%. Based on these results, we propose that the viability of hUC-MSCs can be characterized by these three peaks. And their intensity changes can be understood from the model of excessive reactive oxygen species interacting with the biomacromolecules.