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Detecting undetected HIV-1 variants in African children using degenerate polymerase chain reaction and sequence analyses

โœ Scribed by M. Nanteza; F.C. Kasolo; M. Monze; U.A. Gompels


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
279 KB
Volume
92
Category
Article
ISSN
0035-9203

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## Abstract The detection of HIVโ€1 proviral DMA in children born to seropositive mothers was studied using the polymerase chain reaction with either a radioactive electrophoretic method or a novel procedure that employs colorimetric microwell visualization. Peripheral blood mononuclear cell lysate