𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detecting protein–protein interaction in live yeast by flow cytometry

✍ Scribed by Billy T. Dye; Kathleen Schell; David J. Miller; Paul Ahlquist


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
473 KB
Volume
63A
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


High throughput flow cytometry based yea
✍ Jun Chen; Mark B. Carter; Bruce S. Edwards; Hong Cai; Larry A. Sklar 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 733 KB

## Abstract The analysis of protein‐protein interactions is a key focus of proteomics efforts. The yeast two‐hybrid system (Y2H) has been the most commonly used method in genome‐wide searches for protein interaction partners. However, the throughput of the current yeast two‐hybrid array approach is

Detection of site-specific glycosylation
✍ Deepak Jayakumar; Dhananjay D. Marathe; Sriram Neelamegham 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 284 KB

## Abstract We tested the possibility that we may express unique peptide probes on cell surfaces, and detect site‐specific glycosylation on these peptides using flow cytometry. Such development can enhance the application of flow cytometry to detect and quantify post‐translational modifications in

Sensitive detection technique of myelope
✍ Imamura, Nobutaka 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 55 KB 👁 2 views

This report describes the analysis of culture cells and blast cells separated from the heparinized bone marrow and whole blood of patients with acute leukemias by means of a density-gradient technique (Ficoll-sodium metrizoate d = 1.077 g/cm 3 ). Cell-surface antigens were analyzed by a fluorescence

Detection of protein–protein interaction
✍ Mingyue He; Hong Liu; Martin Turner; Michael J. Taussig 📂 Article 📅 2009 🏛 Elsevier 🌐 English ⚖ 230 KB

We describe a method for identification of protein-protein interactions by combining two cell-free protein technologies, namely ribosome display and protein in situ immobilisation. The method requires only PCR fragments as the starting material, the target proteins being made through cell-free prote