Detecting oxygen consumption in the proximity of Saccharomyces cerevisiae cells using self-assembled fluorescent nanosensors
✍ Scribed by Yina Kuang; David R. Walt
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 349 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
We describe a strategy for the preparation and self‐assembly of fluorescent nanosensors onto Saccharomyces cerevisiae cell surfaces for dynamically measuring oxygen concentration in the proximity of living cells. Amine functionalized polystyrene nanobeads were impregnated with an oxygen‐sensitive ruthenium(II) complex and the beads' surface was coated with polyethylenimine. The resulting nanosensors were assembled on individual S. cerevisiae cells in a controlled manner at physiological pH for continuously monitoring oxygen consumption. This approach exemplifies a general scheme for assembling fluorescent nanosensors on cells for the non‐invasive, reversible, and real‐time measurement of other physiologically relevant processes, such as the efflux of protons and carbon dioxide, or the influx of glucose. Biotechnol. Bioeng. 2007;96: 318–325. © 2006 Wiley Periodicals, Inc.