Desorption of Trichoderma reesei cellulase from cellulose by a range of desorbents

✍ Scribed by D. E. Otter; P. A. Munro; G. K. Scott; R. Geddes

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 620 KB
• Volume: 34
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260340303

✦ Synopsis

The desorption of Trichoderma reesei cellulase from Avicel by a wide range of desorbents was measured. Emphasis was placed on desorption at alkaline pH. A maximum desorption of 65-68% Avicelase activity was achieved by contact with NaOH, pH 10.0, at 40 degrees C for 5 min in the presence of 0.005% Triton X-100 or Tween 80. The design of a suitable desorption process using these conditions is discussed. Glycerol was also effective as a desorbent either alone or in combination with alkali and detergent. However, relatively high concentrations of glycerol were needed and the maximum desorption achieved, 68%, was not significantly greater than that with only alkali and detergent.