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Designing primers from multiple sequences using matchup program to improve detection of hepatitis B virus by polymerase chain reaction

โœ Scribed by So Young Jang; Mi Suk Kim; Min Seok Park; Keon Myung Lee; Hwan Won Chung; Jongsik Chun; Chan Hee Lee


Book ID
107643076
Publisher
The Microbiological Society of Korea
Year
2010
Tongue
English
Weight
120 KB
Volume
48
Category
Article
ISSN
1225-8873

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Three oligonucleotide primer combinations selected from the 5' noncoding, the nucleocapsid and the putative nonstructural regions of the hepatitis C virus genome were compared in a nested polymerase chain reaction assay with respect to sensitivity and specificity for the detection of viral RNA in ch