✦ LIBER ✦

Designed Amyloid β Peptide Fibril—A Tool for High-Throughput Screening of Fibril Inhibitors

✍ Scribed by Gunnar T. Dolphin; Myriam Ouberai; Pascal Dumy; Julian Garcia

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 502 KB
Volume: 2
Category: Article
ISSN: 1860-7179
DOI: 10.1002/cmdc.200700103

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Amyloid β peptide (Aβ) fibril formation is widely believed to be the causative event of Alzheimer’s disease pathogenesis. Therapeutic approaches are therefore in development that target various sites in the production and aggregation of Aβ. Herein we present a high‐throughput screening tool to generate novel hit compounds that block Aβ fibril formation. This tool is an application for our fibril model (Aβ~16–37~Y~20~K~22~K~24~)~4~, which is a covalent assembly of four Aβ fragments. With this tool, screening studies are complete within one hour, as opposed to days with native Aβ~1–40~. A Z′ factor of 0.84±0.03 was determined for fibril formation and inhibition, followed by the reporter molecule thioflavin T. Herein we also describe the analysis of a broad range of reported inhibitors and non‐inhibitors of Aβ fibril formation to test the validity of the system.

📜 SIMILAR VOLUMES

Designed Short Peptides that Form Amyloi

Designed Short Peptides that Form Amyloid-Like Fibrils in Coassembly with Amyloid β-Peptide (Aβ) Decrease the Toxicity of Aβ to Neuronal PC12 Cells

✍ Miho Suzuki; Tsuyoshi Takahashi; Junichi Sato; Masayasu Mie; Eiry Kobatake; Hisa 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 630 KB

Cover Picture: Designed Short Peptides t

Cover Picture: Designed Short Peptides that Form Amyloid-Like Fibrils in Coassembly with Amyloid β-Peptide (Aβ) Decrease the Toxicity of Aβ to Neuronal PC12 Cells (ChemBioChem 11/2010)

✍ Miho Suzuki; Tsuyoshi Takahashi; Junichi Sato; Masayasu Mie; Eiry Kobatake; Hisa 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 961 KB