## Abstract The key to successful and efficient protein purification is the selection of the most appropriate purification techniques and their combination in a logical way to obtain the desired purification in the minimum number of steps. However, the rationalization of protein purification proces
✦ LIBER ✦
Design strategies for integrated protein purification processes: challenges, progress and outlook
✍ Scribed by Beckley K Nfor; Tangir Ahamed; Gijs WK van Dedem; Luuk AM van der Wielen; Emile JAX van de Sandt; Michel HM Eppink; Marcel Ottens
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2008
- Tongue
- English
- Weight
- 400 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0268-2575
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## Abstract An integrated process strategy for the recovery of penicillin acylase was developed, based on precipitation of non‐enzymatic proteins directly from __Escherichia coli__ homogenates or crude extracts using Rolquat (quaternary ammonium salt) and adsorption of the enzyme on Amp‐Seph (3.8 µ