Design of reactive porous polymer supports for high throughput bioreactors: Poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths
✍ Scribed by Shaofeng Xie; Frantisek Svec; Jean M. J. Fréchet
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 153 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/ hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-ben- zoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.