Design of Pulse Sequences for Solvent Suppression and Uniform Excitation. Application to Observing Hemoglobin in Solution and Inside Red Blood Cells

✍ Scribed by B.K. Fetler; V. Simplaceanu; N.S. Vanderven; I.J. Lowe; C. Ho

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 864 KB
• Volume: 101
• Category: Article
• ISSN: 1064-1866
• DOI: 10.1006/jmrb.1993.1003

✦ Synopsis

A general method of selective excitation design for shaped pulses and pulse sequences is presented, based on analysis of the derivatives of (M_{+}(\Delta \omega)), where (M_{+})is the transverse magnetization and (\Delta \omega) is the resonance-offset frequency. We use this method to derive pulse sequences which suppress the solvent resonance at one offset frequency and produce uniform-phase and -amplitude excitation at another offset frequency. Two families of pulse sequences are presented: small-flip-angle asymmetric pulse sequences and large-flip-angle symmetric pulse sequences. Experimental application of the pulse sequences is given for observing 'H NMR resonances of carbonmonoxyhemoglobin and deoxyhemoglobin (deoxy-Hb) in aqueous solution and deoxy-Hb inside intact red blood cells. & 1993 Academic Press. Inc.