Design of an automated immunosensor device for fast detection of Hantavirus in mice blood

✍ Scribed by S. Yelleti; E. Wilkins

• Publisher: Elsevier Science
• Year: 2004
• Tongue: English
• Weight: 481 KB
• Volume: 97
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/j.snb.2003.08.028

✦ Synopsis

A small portable automated device for detection of Hantavirus infection specifically antibodies against Sin Nombre Virus (SNV) in human and mice blood samples, that is reliable, sensitive and reproducible under field conditions has been developed. The system is developed using a small peristaltic pump, solenoid valves and a liquid crystal display all controlled by a programmable microcontroller. The size of the device has been reduced to 21.5 cm × 10.5 cm × 4.5 cm. The device can be easily adapted to detect different kinds of virus infections, like smallpox, HIV, and others. The device analysis time has been optimized to be less than 35 min. The output is qualitative to indicate whether the blood sample is positive (infected) or negative without specifying the concentration of SNV antibodies. A flow-through sandwich scheme amperometric immunoassay using highly dispersed carbon particles as the immunosorbent and also as the working electrode is used.