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Design of Allele Specific PCR for Rapid Detection of CYP3A5 (A6986G) and Mdr-1 (C3435T) Polymorphisms

✍ Scribed by Tester F. Ashavaid; Himanshu S. Raje; Bharat V. Shah; Swarup A. Shah


Book ID
107646959
Publisher
Association of Clinical Biochemistry of India
Year
2010
Tongue
English
Weight
293 KB
Volume
26
Category
Article
ISSN
0970-1915

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C1236T, G2677T/A, and C3435T polymorphism of the multidrug resistance (MDR1) gene have substantial impact on expression or activity of P-glycoprotein (Pgp). We developed new methods based on amplification refractory mutation system (ARMS) to detect these polymorphisms. Tetra-primers amplification in