✦ LIBER ✦

Design of a Silicon Based Amperometric Microbiosensor Involving NAD-Dependent Dehydrogenase

✍ Scribed by Hélène Tap; Pierre Gros; Anne-Marie Gué

Book ID: 101314494
Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 239 KB
Volume: 11
Category: Article
ISSN: 1040-0397
DOI: 10.1002/(sici)1521-4109(199909)11:13<973::aid-elan973>3.0.co;2-3

No coin nor oath required. For personal study only.

✦ Synopsis

An electrochemical microbiosensor involving NAD-dependent dehydrogenase has been designed and developed using silicon technology. The sensor is based on a weakly polarized two-gold microelectrode system without any reference electrode. The process allowed mass production of miniaturized devices. An enzymatic solution including diaphorase and NAD-dependent dehydrogenase was con®ned in the microstructure by means of a semi-permeable membrane. The biocompatibility of the various materials was veri®ed and the gold electrodes were electrochemically characterized. The assay of D-lactic acid was taken as an example using D-lactate dehydrogenase. The sensitivity and detection limit of the microbiosensor were 8 mA mM À1 cm À2 and 0.01 mM respectively. Its lifetime was 3 weeks.

📜 SIMILAR VOLUMES

ChemInform Abstract: Amperometric Biosen

ChemInform Abstract: Amperometric Biosensors Based on NAD(P)-Dependent Dehydrogenase Enzymes

✍ M. J. LOBO; A. J. MIRANDA; P. TUNON 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 24 KB

Development of an amperometric alcohol s

Development of an amperometric alcohol sensor based on immobilized alcohol dehydrogenase and entrapped NAD+

✍ S. Miyamoto; T. Murakami; A. Saito; J. Kimura 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 553 KB

Isolation and characterization of a benz

Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in d-phenylglycine metabolism in Pseudomonas stutzeri ST-201

✍ Choedchai Saehuan; Theerasak Rojanarata; Suthep Wiyakrutta; Michael J. McLeish; 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 787 KB

Cloning of a cDNA encoding a NAD-depende

Cloning of a cDNA encoding a NAD-dependent formate dehydrogenase involved in oxalic acid metabolism from the white-rot fungus Ceriporiopsis subvermispora and its gene expression analysis

✍ Tomoki Watanabe; Tadashi Fujiwara; Toshiaki Umezawa; Mikio Shimada; Takefumi Hat 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 465 KB

A reagentless amperometric electrode bas

A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD+, and mediator containing polymer for D-lactic acid analysis. II. On-line monitoring of fermentation process

✍ Hun-Chi Shu; Lo Gorton; Björn Persson; Bo Mattiasson 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 447 KB 👁 3 views