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Design of a Molecular Beacon DNA Probe with Two Fluorophores

✍ Scribed by Peng Zhang; Terry Beck; Weihong Tan


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
177 KB
Volume
40
Category
Article
ISSN
0044-8249

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✦ Synopsis


Fluorescence resonance energy transfer between two fluorophores (F and F ) attached to the two ends of a molecular beacon DNA probe containing a hairpin structure can be used for quantitative DNA/RNA studies. Concentrations of target-DNA as low as 1.7Γ—10  M could be determined with a commercial spectrometer by using coumarin and 6-carboxyfluorescein as the fluorophores. Measurements on the FΓΆrster energy transfer distance for the donor/acceptor pair can also be carried out using these DNA probes.


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