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Design of a Mechanism-Based Probe for Neuraminidase To Capture Influenza Viruses

✍ Scribed by Chun-Ping Lu; Chien-Tai Ren; Yi-Ning Lai; Shih-Hsiung Wu; Wei-Man Wang; Jean-Yin Chen; Lee-Chiang Lo


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
130 KB
Volume
117
Category
Article
ISSN
0044-8249

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A fluorometric procedure for quantitating the amount of N-acetylneuraminic acid enzymatically released by the neuraminidase activity from N-acetylneuraminyl-lactose (sialyl-lactose) has been developed. The liberated lactose is hydrolyzed with beta-galactosidase, and the released galactose is oxidize